Fig. 2

Effect of cortisone on yEGFP expression in the strain RVY97. a Increased cortisone concentrations led to an increase of yEGFP expression in a concentration dependent manner. The treated RVY97 population (10,000 events) was analysed by flow cytometry and the results are expressed in percentage of RVY97 cells showing yEGFP fluorescence. Data shown represent means ± standard deviations of three independent experiments. b Fluorescence microscopy of strain RVY97 showed that cortisone treated cells displayed a strong fluorescent response to the hormone. c Flow cytometry analysis of the strain RVY97 cultivated with DMSO (upper panel) and 250 μM cortisone (lower panel) enabled quantitative analysis of the hormone response of this specific strain. The gate highlights the RVY97 population positive for the yEGFP expression. All analyses were carried out 6 h after the addition of DMSO and cortisone, respectively. (a.u.): arbitrary unit