Fig. 1

Schematic representation of the screening strain RVY97. In strain RVY97 the cortisone provided in the growth medium is converted to cortisol inside the cell by the enzyme 11β-HSD1. Cortisol binds the GR inducing assembly of an active complex that translocates to the nucleus. Through binding of the complex to the GRE, expression of the yEGFP reporter protein is triggered. In the presence of 11β-HSD1 inhibitors, cortisol is not produced, the GR is inactive and yEGFP not expressed