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Cloning into pGREG526
|
|
SkARO10-aF
|
CCTAGTACGGATTAGAAGCCGCCGAGCGGGTGACAACTTTTGATTTGTTCCCCGC
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|
SkARO10-aR
|
GCGTGACATAACTAATTACATGACTCGAGGTCGACAAAGACAAAATCGGCGGC
|
|
ScARO10-F
|
CCTAGTACGGATTAGAAGCCGCCGAGCGGGTGACAATCTCTTAGGCATGCTCTTGG
|
|
ScARO10-R
|
GCGTGACATAACTAATTACATGACTCGAGGTCGACTATAATTGCGCCCACAAGTTTC
|
|
ARO10 deletion cassette
|
|
TaARO10-NAT1-F
|
ATGGCACCTGTTACAATTGAAAAGTTCGTAAATCAAGAAGGGTGTTTAGGTCGATGCCATC
|
|
TaARO10-NAT1-R
|
CTATTTTTTATTTCTTTTAAGTGCCGCTGCTTCAACCATGGGATGGCGGCGTTAGTATCG
|
|
Integration fragment
|
|
pGSkARO10f
|
TAAAGTTTATTTACAAGATAACAAAGAAACTCCCTTAAGCATGACGCCTGTTACAATTAA
|
|
pGScARO10f
|
TAAAGTTTATTTACAAGATAAC
|
|
pG-ARO10-R
|
ACAATTGGTAGCAGTGTTTTATAATTGCGCCCACAAGTTTCTCACTATAGGGCGAATTGG
|
|
Diagnostic
|
|
T73AR10-UF
|
ATCTCTTAGGCATGCTCTTGG
|
|
K2
|
GGGACAATTCAACGCGTCTG
|
|
K3
|
CCTCGACATCATCTGCCC
|
|
ScARO10-R1
|
GAAGTCACCAGGAACACCG
|
|
SkARO10-R1
|
CATTGGAAACAAGGTGCGG
|
