Table 3 Primers used in this study
Primer | Sequence 5′–3′ |
|---|---|
Cloning into pGREG526 | |
SkARO10-aF | CCTAGTACGGATTAGAAGCCGCCGAGCGGGTGACAACTTTTGATTTGTTCCCCGC |
SkARO10-aR | GCGTGACATAACTAATTACATGACTCGAGGTCGACAAAGACAAAATCGGCGGC |
ScARO10-F | CCTAGTACGGATTAGAAGCCGCCGAGCGGGTGACAATCTCTTAGGCATGCTCTTGG |
ScARO10-R | GCGTGACATAACTAATTACATGACTCGAGGTCGACTATAATTGCGCCCACAAGTTTC |
ARO10 deletion cassette | |
TaARO10-NAT1-F | ATGGCACCTGTTACAATTGAAAAGTTCGTAAATCAAGAAGGGTGTTTAGGTCGATGCCATC |
TaARO10-NAT1-R | CTATTTTTTATTTCTTTTAAGTGCCGCTGCTTCAACCATGGGATGGCGGCGTTAGTATCG |
Integration fragment | |
pGSkARO10f | TAAAGTTTATTTACAAGATAACAAAGAAACTCCCTTAAGCATGACGCCTGTTACAATTAA |
pGScARO10f | TAAAGTTTATTTACAAGATAAC |
pG-ARO10-R | ACAATTGGTAGCAGTGTTTTATAATTGCGCCCACAAGTTTCTCACTATAGGGCGAATTGG |
Diagnostic | |
T73AR10-UF | ATCTCTTAGGCATGCTCTTGG |
K2 | GGGACAATTCAACGCGTCTG |
K3 | CCTCGACATCATCTGCCC |
ScARO10-R1 | GAAGTCACCAGGAACACCG |
SkARO10-R1 | CATTGGAAACAAGGTGCGG |