Table 1 List of primers used in this study
Name | 5´–3´ Sequence |
|---|---|
PlacSynth_SacI_EcoRI_F | GATGACGAGCTC GAATTCTGGTCTTTATTCTTCAA |
M13_R_NheI | CGACGAGCTAGCAGCCAGGAAACAGCTATGACC |
mCherry_RBS_XbaI | GCTGCTTCTAGAAAGGAGGAAATTATACATGTTATCAAAGGGTGAAGAAG |
mCherry_R_BamHI | CGTCGTGGATCCTTATCACTTGTATAATTCATCCATACC |
Tldh_amp_R_PstI | CTGCTGCTGCAGAAAAAGATTAAAAAAGCCGCTGC |
mCherry_seq_R | TGGACGACCTTCACCTTCAC |
mCherry_seq_F | AACGTATGTACCCAGAAGATG |
CAT_seq2_back | TACATCATTCTGTTTGTGATGG |
B_mega_XylOP_out_F | AACATATAAACAGCCAGTTGCC |
B_mega_XylOP_R(SpeI, ScaI, BamHI) | GTAGTAGGATCC AGT ACTAGTTTCCCCCTTTGATTTAAGTG |
mCherry_w/o_RBS_XbaI | CGTCGTTCTAGAATGTTATCAAAGGGTGAAGAAGATAAC |
p256_miniori_for | CATCATAAGCTTCCCGCACGCATAGCGGTGC |
B_mega_XylOP_F_MfeI, KpnI | GTAGTACAATTG GGTACCAAGGTGAGGGTGGAGACAG |
Bmega_XylR_newRBS_XbaI_Phos_R | GTATAATTTCCTCCTTTCTAGAAGTGAACAAGTTTATCCAT |
mCherry_Phos_F | ATGTTATCAAAGGGTGAAGAAG |
B_mega_XylOP_seq_F | CAATTCCGATATTAATACTGATG |
B_mega_XylOP_seq_R | CTAGTCGGAATAGGAATTTGTG |
LacI_Lplant_F_SacI | AGCAGCGAGCTCCCTAATAGAACTGCGGTGGTC |
LacI_Lplant_R_XbaI | AGCAGCTCTAGAAACTTAATTACGCTTTAACAGC |
lacR_Gal_seq_R | AATTGAAGTGATGCGGGTCTG |
lacR_Gal_seq_F | AATTGCGCCAGCTAACACCC |
T7_RNAP_Lp_RBS | CAGCAGTCTAGATCCTAAAGGAGG |
T7_RNAP_Lp_Term_R_SalI | CAGCAGGTCGACTTGATATACAAAAAAGG |
M13_2_F | TTGTAAAACGACGGCCAGTG |
T7-Promoter_SacI | GCTGCTGAGCTCAGATCGATCTCGATCCCGCG |
T7-Terminator_SalI | GCTGCTGTCGACTCCGGATATAGTTCCTCCTTTC |
ery_back_KasI | CATCATGGCGCCTCCGATTGCAGTATAAATTTAACG |
oripE194_seq_back | AATCAAATCGGTATAAATCTGAC |
Ery_F_NheI | CATCATGCTAGCTCCGATTGCAGTATAAATTTAACG |
Pempty_SacI_R | TAGTAGTCTA GA GCTCGAATTCACTGGCCGTCG |
mCherry_RBS_SacI_F | GCTGCTGAGCTCAAGGAGGAAATTATACATGTTATCAAAGGGTGAAGAAG |