Fig. 7

Expression and growth comparison of inducible promoters to constitutive promoter. BioLector^® micro-fermentation measurement for 23 h in selective MRS medium with glucose with low pH FlowerPlate at 30 °C (individual values). Dotted vertical line indicates sampling point (200 µl) for semi-quantitative Western blot, 13 h after start, followed by ongoing measurement, therefore OD₆₀₀ curves are shifted after sampling. Arrow indicates induction time point (or absence of inducer). Solid line mCherry under control of constitutive P₁₁ promoter (pCDLbu1ΔEc_P₁₁_mCherry), –x-: T7 RNA polymerase based dual plasmid system induced with 1 mM IPTG, square P_xylA induced with 2 % xylose, triangle P_lacSynth induced with 1 mM IPTG, circle P_lacA induced with 2 % lactose. a specific expression levels of mCherry under control of inducible promoters with respective inducers. b corresponding calculated OD₆₀₀ values