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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Evaluation of novel inducible promoter/repressor systems for recombinant protein expression in Lactobacillus plantarum

Fig. 2

Maps of plasmids used in this study. Annotations and relevant restriction sites are indicated. a pCDLbu1 initial vector backbone (highlighted region are E. coli specific sequences); b pCDblu1_PxylA_mCherry; c pCDLbu1_PlacA_mCherry; d pCDLbu1_PlacSynth_mCherry; e pCDLbu1ΔEc_P11_mCherry (constitutive P11 promoter; internal reference plasmid described by Tauer and colleagues [47]); The term ‘ΔEc’ indicates removal of E. coli specific sequences which are highlighted in plasmid A. f pCD256_PlacSynth_mCherry; g pCD256_PlacSynth_T7RNAP; h pCDLbu1ΔEc_PT7_mCherry _TT7_Ery. OripCDLbu1 and miniori256: origins of replication (ori) for L. plantarum 3NSH; pMB1ori: ori for replication in E. coli; CAT chloramphenicol acetyltransferase gene; Amp ampicillin resistance gene; Ery ermI gene encoding resistance to erythromycin; P promoter; T terminator of transcription. Subscripted characters are specifications. Important restriction sites are indicated

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