De novo production of raspberry ketone during wine fermentation. A strain containing the Rhodosporidium
toruloides PAL (RtPAL) and cinnamate-4-hydroxylase from Arabidopsis
thaliana (AtC4H) as separate ORFs in addition to the P. crispum coumarate CoA ligase 2 and benzalacetone synthase from R.
palmatum ORFs fused by rigid linker (Pc4CL2-r-RpBAS) was fermented in Chardonnay grape juice in either airlock flasks (anaerobic) or standard flasks (aerobic) until dryness and assessed for raspberry ketone production via LC/MS