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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Heterologous production of raspberry ketone in the wine yeast Saccharomyces cerevisiae via pathway engineering and synthetic enzyme fusion

Fig. 2

Biosynthesis of raspberry ketone from p-coumaric acid during anaerobic fermentation. Codon optimized genes encoding coumarate CoA ligase (4CL) from either A. thaliana (At4CL1, pink) or P. crispum (Pc4CL2, blue) and benzalacetone synthase (BAS) from R. palmatum (RpBAS) were integrated at the HO locus of S. cerevisiae as either two independent genes or as a single ORF fused by either a flexible (f) or rigid (r) amino acid linker. Levels of raspberry ketone were assessed following 5 days growth at 22 °C in air-lock flasks in synthetic grape juice medium, supplemented with 3 mM p-coumaric acid and assessed for raspberry ketone production via LC/MS

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