Enhancing full-length antibody production by signal peptide engineering

Table 3 The effects of signal peptide hydrophobicity on hu5D5 heavy chain processing

	Signal peptide	ΔHydro	Mature HC %	Signal peptide	ΔHydro	Mature HC %
HC only	ssSTII1	–	29	ssDsbA1	–	84
	ssSTII1 S-13L	+1.24	70	ssDsbA1 L-9S	−1.24	38
	ssSTII1 S-13I	+1.56	58	ssDsbA1 L-9Q	−1.91	20
	ssSTII1 S-13Y	+0.44	22	ssDsbA1 L-9A	−0.44	60
	ssSTII1 A-18L A-14L S-13L	+2.12	65	ssDsbA1 L-9I	+0.32	82
LC and HC co-expressed	ssSTII1	–	45	ssDsbA	–	86
	ssSTII S-13L	+1.24	67	ssDsbA1 L-9S	−1.24	39
	ssSTII S-13I	+1.56	66	ssDsbA1 L-9I	+0.32	81

ΔHydro represents the change in total hydrophobicity resulting from the amino acid variants

ISSN: 1475-2859