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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Enhancing full-length antibody production by signal peptide engineering

Fig. 3

The effects of signal peptide hydrophobicity on full-length hu5D5 production. For all the constructs, light chain was fused to ssSTII0.3 and heavy chain was fused to ssSTII1, ssDsbA1, ssPhoA1, ssMalE1, ssSTII1 S-13L, ssSTII1 S-13I, ssDsbA1 L-9S, or ssDsbA L-9I. Top panel the non-reduced soluble protein samples from the cell lysates were separated by electrophoresis followed by Western blot analysis. Heavy chain-containing species are marked based on the apparent molecular weight. Middle panel Western blot of soluble heavy chain in the periplasm. The periplasmic protein samples were reduced with 0.2 M DTT. Bottom panel Western blot of reduced total heavy chain in the whole cell lysates. All the blots were probed with the HRP-conjugated anti-Fc antibody

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