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Table 2 CYP76AH24 kinetic parameters with different substrates

From: Production of the forskolin precursor 11β-hydroxy-manoyl oxide in yeast using surrogate enzymatic activities

Substrate

K M (μM)

k cat (min−1)

k cat/K M (×103 min−1 M−1)

Source

Manoyl oxide

11.6 ± 1.06

3.65 ± 0.12

315

This study

Ferruginol

45.84 ± 9.37

6.11 ± 0.45

133

Ref. [26]

Miltiradiene

72.63 ± 43.19

1.97 ± 0.72

27.1

Ref. [26]