Skip to main content
Fig. 5 | Microbial Cell Factories

Fig. 5

From: Production of the forskolin precursor 11β-hydroxy-manoyl oxide in yeast using surrogate enzymatic activities

Fig. 5

Steady-state kinetic analysis of the oxidation of manoyl oxide by CYP76AH24. The enzymatic activity of CYP76AH24 was evaluated using varying concentration (1–75 μM) of manoyl oxide substrate and 80 pmol of enzyme. The produced 11β-hydroxy-manoyl oxide was quantified by CG–MS analysis using purified compound as standard. Quantification of CYP76AH24 enzyme concentration was performed by measuring the binding of CO to the reduced form of the purified enzyme (450 nm peak), according to [47]. The differential spectrum of the CO-treated enzyme preparation is shown in the inset

Back to article page

Microbial Cell Factories

ISSN: 1475-2859

Contact us