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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Production of the forskolin precursor 11β-hydroxy-manoyl oxide in yeast using surrogate enzymatic activities

Fig. 3

Diagrammatic illustration of configuration of the yeast platform for the production manoyl oxide and 11β-hydroxy-manoyl oxide. a The fusion between the class II diTPS, CcCLS, responsible for 8OH-CPP formation and the yeast variant Erg20p(F96C), engineered to synthesize GGPP, under uracil selection (U) was co-expressed in yeast cells with the class I diTPS, SpMilS, under histidine (H) selection, for the production of manoyl oxide. b Introduction of CYP76AH24 in the above system, under leucine (L) selection, enabled oxidation of manoyl oxide to 11β-hydroxy-manoyl oxide

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Microbial Cell Factories

ISSN: 1475-2859

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