Table 2 Mass spectrometry (MS) analysis of differentially expressed proteins between E. coli MG1655 and MG12345 (using MG12345 as the standard)
No | Accession number | Gene name | Protein name | Mr | PI | Protein sequence coverage (%) | Difference fold |
|---|---|---|---|---|---|---|---|
1 | I16 | acpH | Acyl carrier protein phosphodiesterase | 22,937 | 5.53 | 46 | 37.05 |
2 | I17 | aroC | Chorismate synthase | 38,520 | 5.61 | 23 | 20.71 |
3 | I18 | pyrC | Dihydroorotase | 38,983 | 5.68 | 41 | 14.7 |
4 | I19 | glnA | Glutamine synthetase, type I | 51,230 | 5.27 | 44 | 0.14 |
5 | I22 | aldA | Lactaldehyde dehydrogenase | 52,362 | 5.07 | 30 | 0.25 |
6 | I23 | potD | Spermidine/putrescine ABC transporter substrate-binding protein | 40,899 | 5.66 | 11 | 69.67 |
7 | I24 | cpdB | 2′,3′-cyclic-nucleotide 2′-phosphodiesterase | 70,799 | 5.52 | 21 | 0.17 |
8 | J1 | dppA | Periplasmic dipeptide transport protein | 60,483 | 6.36 | 28 | 0.11 |
9 | J2 | aceE | Pyruvate dehydrogenase | 62,256 | 5.86 | 26 | 7.39 |
10 | J3 | gadA | Glutamate decarboxylase alpha | 55,736 | 5.37 | 26 | 7.01 |
11 | J5 | talB | Transaldolase | 35,838 | 5.75 | 31 | 15.65 |
12 | J6 | dapD | 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase | 30,045 | 5.56 | 47 | 0.023 |
13 | J8 | menB | Naphthoate synthase | 32,737 | 6.32 | 25 | 57.61 |
14 | J9 | glmM | Phosphoglucosamine mutase | 47,830 | 5.61 | 25 | 32.56 |
15 | J10 | mrp | Mrp protein | 41,222 | 6.05 | 19 | 10.05 |
16 | J11 | glpQ | Periplasmic Glycerophosphodiester Phosphodiesterase | 40,617 | 5.35 | 32 | 0.156 |
17 | J12 | potE | Periplasmic putrescine binding protein | 40,900 | 5.67 | 18 | 0.09 |
18 | J13 | aph | Aminoglycoside 3′-phosphotransferase | 29,315 | 4.64 | 62 | 9.45 |
19 | J15 | pykF | Pyruvate kinase I | 50,675 | 5.77 | 26 | 5.86 |
20 | J16 | aspA | Aspartate ammonia-lyase | 52,920 | 5.25 | 12 | 0.055 |
21 | J17 | tnaA | Tryptophanase | 53,081 | 6 | 21 | 12.36 |
22 | J19 | oppA | Periplasmic oligopeptide-binding protein | 46,735 | 5.5 | 39 | 0.02 |