Table 1 Strains and plasmids
Name | Characteristic | Source |
|---|---|---|
Strain | ||
 MG1655 | Wild type of Escherichia coli | Laboratory collection |
 MG001 | MG1655 ΔtnaA::KanR | This work |
 MG003 | MG1655 Δggt::KanR | This work |
 MG004 | MG1655 ΔpepT::KanR | This work |
 MG005 | MG1655 ΔglgB:: KanR |  |
 MG012 | MG1655 ΔtnaAΔsdaA::KanR | This work |
 MG034 | MG1655 ΔggtΔpepT::KanR | This work |
 MG123 | MG1655 ΔtnaAΔsdaAΔggt::KanR | This work |
 MG1234 | MG1655 ΔtnaAΔsdaAΔggtΔpepT::KanR | This work |
 MG12345 | MG1655 ΔtnaAΔsdaAΔggtΔpepTΔglgB::KanR | This work |
 ZJ000 | MG1655 harboring pTrc99a-as | This work |
 ZJ001 | MG001 harboring pTrc99a-as | This work |
 ZJ012 | MG012 harboring pTrc99a-as | This work |
 ZJ123 | MG123 harboring pTrc99a-as | This work |
 ZJ1234 | MG1234 harboring pTrc99a-as | This work |
 ZJ12345 | MG12345 harboring pTrc99a-as | This work |
Plasmid | ||
 pKD4 | kan cassette template, KanR | [32] |
 pKD46 | λ Red recombinase under arabinose-inducible araBAD promoter, temperature-conditional replicon, AmpR | [32] |
 pCP20 | FLP recombinase expression, temperature-conditional replicon, AmpR and CmR | [32] |
 pET28a-as | pET28a carries a bifunctional glutathione synthetase (gshF) from Actinobacillus succinogenes, KanR | Yang et al. (to be published) |
 pTrc99a | Cloning vector, AmpR | Laboratory collection |
 pTrc99a-as | pTrc99a carries a bifunctional glutathione synthetase (gshF) from Actinobacillus succinogenes, AmpR | This work |