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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Protein aggregation and membrane lipid modifications under lactic acid stress in wild type and OPI1 deleted Saccharomyces cerevisiae strains

Fig. 4

Second derivatives of the FTIR absorption spectra of S. cerevisiae BY4741 cells, in the absence and in the presence of lactic acid: stationary phase. Cells were grown in shake flasks in minimal (YNB) medium with 2 % w/v glucose in the absence and in the presence of 46 g/L of lactic acid (LA) at pH3. FTIR analysis was performed at 40 h after the inoculation, corresponding to the stationary phase of growth. a: amide I band; b: vibrational modes mainly due to lipid CH2/CH3 and to phosphate groups, c: stretching modes from lipid hydrocarbon tails. In a and b second derivative spectra have been normalized to the tyrosine band at ~1516 cm−1, while in c spectra have been normalized at the CH3 band at ~2959 cm−1

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