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Fig. 3 | Microbial Cell Factories

Fig. 3

From: A novel esterase from a marine mud metagenomic library for biocatalytic synthesis of short-chain flavor esters

Fig. 3

SDS-PAGE analysis of soluble lysates of lipolytic enzymes and the purified esterase EST4. Lane M, molecular weight protein marker (Thermo Scientific, Cat. No: 26610); lane 1, soluble lysates of E. coli Top10Fʹ/pLLP-OmpA, as negative control; lane 2, soluble lysates of E. coli BL21/pET28a, as negative control; lane 3, EST1; lane 4, EST2; lane 5, EST3; lane 6, EST4; lane 7, EST5; lane 8, purified EST4 (34 kDa). The arrows indicate expected proteins from recombinant E. coli

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Microbial Cell Factories

ISSN: 1475-2859

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