Skip to main content
Fig. 4 | Microbial Cell Factories

Fig. 4

From: Automation of a Nile red staining assay enables high throughput quantification of microalgal lipid production

Fig. 4

Gravimetric calibration of the lipid assay. The Nile red staining deviated biomass specific lipid fluorescence is linearly correlated with the fraction of intracellular lipids measured by extraction and subsequent gravimetric analysis. Cells with a biovolume of 0.15 µL mL−1 were stained for 3 h at 40 °C in 25 % (v v−1) DMSO with 1 mg L−1 Nile red. Error bars of the Nile red assay deviated from analytical replicates (n = 5). Error bars of the gravimetric measurement from technical replicates (n = 3)

Back to article page