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Table 2 Time and costs of performing traditional plate PTVA and liquid PTVA for both L12 and L24 with Zeocin and G418 selection [28]

From: Liquid PTVA: a faster and cheaper alternative for generating multi-copy clones in Pichia pastoris

 

Zeocin

G418

Plate PTVA

L12 PTVA

L24 PTVA

Plate PTVA

L12 PTVA

L24 PTVA

Time (days)

45

8 ½

12

45

8 ½

12

Cost (USD)

67.47

32.18

32.18

39.82

18.56

18.56

  1. Costs were determined based on prices on the Thermo Fisher Scientific website