Fig. 1From: Liquid PTVA: a faster and cheaper alternative for generating multi-copy clones in Pichia pastoris Plate and liquid PTVA methodology. Schematic representation of the method for generating multi-copy clones by traditional plate PTVA (left) and liquid PTVA (right) changing medium either every 12 or 24 h. Unless otherwise stated concentrations are for both Zeocin and G418 selectionBack to article page