Table 1 Plasmids and strains used in the study
Strain and plasmids | Relevant genetic features | Description | Reference |
|---|---|---|---|
Plasmids | |||
YIplac211 | URA3 | [39] | |
YIpOB7 | URA3 PGK1p-XYL2-PGK1t, TDH3p-ADH1t | [31] | |
YIpOB7-SADH | URA3 PGK1p-SADH-PGK1t | This study | |
YIpOB7-SADH-VAMT | URA3 PGK1p-SADH-PGK1t, TDH3p-VAMT-ADH1t | This study | |
S. cerevisiae strains | |||
TMB4132 | CEN.PK2-1C his3::YIpJK01 trp1::TRP1 leu2::LEU2 ura3 - | [21] | |
TMB4133 | CEN.PK2-1C his3::YIpJK01 gpd1::TRP1 gpd2:LEU2 ura3 - | [21] | |
TMB4140 | TMB4132 ura3:: YIplac211 | Empty plasmid | [21] |
TMB4144 | TMB4133 ura3:: YIplac211 | Empty plasmid, gpd1Δgpd2Δ | [21] |
TMB4160 | TMB4132 ura3::YIpOB7-SADH | SADH | This study |
TMB4161 | TMB4133 ura3::YIpOB7-SADH | SADH, gpd1Δgpd2Δ | This study |
TMB4162 | TMB4132 ura3::YIpOB7-SADH-VAMT | VAMT and SADH | This study |
TMB4163 | TMB4133 ura3::YIpOB7-SADH-VAMT | VAMT and SADH, gpd1Δgpd2Δ | This study |