Fig. 2

Whole-cell bioconversion of racemic 1-phenylethylamine to (R)-1-phenylethylamine and (S)-1-phenylethanol with cells over-expressing both SADH and VAMT encoding genes. a Conversion (%) of (S)-1-phenylethylamine, and b concentration (mM) of (S)-1-phenylethanol during whole-cell biotransformation of racemic 1-phenylethylamine. Reactions were catalysed using recombinant S. cerevisiae strains over-expressing SADH from R. erythropolis and VAMT from C. chinense, and having native (TMB4162) (filled symbols) or deleted (TMB4163) (open symbols) glycerol-3-phosphate dehydrogenase (GPD) activity. Reaction conditions were as follows: 5 g (dw)/l recombinant S. cerevisiae, 5 mM racemic 1-phenylethylamine, 50 g/l glucose, in a defined mineral medium buffered to pH 7. S. cerevisiae was grown in aerobic batch mode in stirred tank reactors with defined media with glucose as carbon and energy source and harvested by centrifugation in early stationary phase. Mean values and standard deviations are calculated from two different whole-cell bioconversions