Skip to main content
Fig. 1 | Microbial Cell Factories

Fig. 1

From: Increased availability of NADH in metabolically engineered baker’s yeast improves transaminase-oxidoreductase coupled asymmetric whole-cell bioconversion

Fig. 1

Whole-cell bioconversion of acetophenone to (S)-1-phenylethanol. Reactions were catalysed by engineered S. cerevisiae strains, over-expressing a SADH from R. erythropolis (TMB4160 and TMB4161); or, b SADH from R. erythropolis in series with VAMT from C. chinense (TMB4162 and TMB4163). Reaction conditions were as follows: 5 g (dw)/l recombinant S. cerevisiae, 5 mM (a) or 7.5 mM (b) acetophenone, 50 g/l glucose, in defined mineral medium buffered to pH 7. Prior to the reactions, cells were grown in aerobic batch mode with defined medium with glucose as carbon and energy source and harvested by centrifugation in early stationary phase. Symbols: (a) filled squares, TMB4160 (control, SADH); open squares, TMB4161 (gpd1Δgpd2Δ, SADH). (b) filled triangles TMB4162 (control, VAMT and SADH), open triangles (gpd1Δgpd2Δ, VAMT and SADH). Mean values and standard deviations are calculated from two different whole-cell bioconversions

Back to article page

Microbial Cell Factories

ISSN: 1475-2859

Contact us