Proteomics analysis of high lipid-producing strain Mucor circinelloides WJ11: an explanation for the mechanism of lipid accumulation at the proteomic level

Tang, Xin; Zan, Xinyi; Zhao, Lina; Chen, Haiqin; Chen, Yong Q.; Chen, Wei; Song, Yuanda; Ratledge, Colin

doi:10.1186/s12934-016-0428-4

Table 2 Identification of differentially expressed proteins under N deficiency in M. circinellodies WJ11 by MALDI-TOF/TOF analysis

From: Proteomics analysis of high lipid-producing strain Mucor circinelloides WJ11: an explanation for the mechanism of lipid accumulation at the proteomic level

Spot	Accession	Protein name	Theoretical MW (Da)/pI	MASCOT score	No. matched	Coverage (%)	Fold change 24 h/6 h	Fold change 60 h/6 h
Nitrogen metabolism
1	gi\|511001426	Glutamine synthetase	39,973/5.61	73	2 (0)	6	14.49	5.58
2	gi\|511003862	Glutamine synthetase	40,412/5.61	102	2 (2)	11	15.04	2.79
3	gi\|511008284	Saccharopepsin	44,921/4.81	243	5 (2)	17	37.86	60.50
4	gi\|511001898	Saccharopepsin	44,736/4.91	129	3 (0)	8	36.84	91.88
Amino acid metabolism
5	gi\|511002358	Acetolactate synthase	73,772/8.13	297	5 (1)	10	0.02	1.03
6	gi\|511004294	Dihydroxy-acid dehydratase	64,148/6.27	432	8 (4)	11	0.55	0.82
7	gi\|511002359	Ketol-acid reductoisomerase	44,298/7.72	562	9 (3)	27	0.10	0.17
8	gi\|511004962	Branched-chain amino acid aminotransferase	43,753/6.62	474	6 (3)	19	0.38	0.59
9	gi\|511010054	Acetylornithine aminotransferase	49,342/8.32	123	4 (1)	15	0.75	0.37
10	gi\|511006376	Asparagine synthetase	64,754/5.81	88	3 (0)	8	0.15	0.80
11	gi\|511005296	Saccharopine dehydrogenase	41,899/5.36	471	12 (3)	35	0.38	0.29
12	gi\|511002126	Homoisocitrate dehydrogenase	41,047/6.28	102	3 (0)	10	0.29	0.15
13	gi\|511009910	Serine hydroxymethyltransferase 2	51,589/6.53	193	8 (2)	24	0.39	0.31
14	gi\|511001743	Serine hydroxymethyltransferase, mitochondrial	55,707/8.97	109	4 (1)	11	0.30	0.22
15	gi\|511000252	Glycine cleavage system T protein	43,694/8.77	82	4 (0)	11	0.18	0.47
16	gi\|511004260	3-Deoxy-7-phosphoheptulonate synthase	38,738/6.46	239	4 (1)	9	0.20	0.10
17	gi\|511010625	S-Adenosylmethionine synthase	42,413/5.67	70	1 (1)	3	5.39	3.55
18	gi\|511010625	S-Adenosylmethionine synthase	42,413/5.67	106	1 (1)	3	3.22	1.41
Carbon metabolism
19	gi\|511002640	Fructose-bisphosphate aldolase	39,791/5.36	145	2 (2)	11	1.64	2.99
20	gi\|511002640	Fructose-bisphosphate aldolase	39,791/5.36	236	3 (1)	11	1.55	1.51
21	gi\|511008895	Glyceraldehyde-3-phosphate dehydrogenase 1	35,588/6.09	88	3 (0)	10	2.77	7.56
22	gi\|511008895	Glyceraldehyde-3-phosphate dehydrogenase 1	35,588/6.09	106	2 (0)	5	8.24	13.63
23	gi\|511008895	Glyceraldehyde-3-phosphate dehydrogenase 1	35,588/6.09	394	5 (3)	15	2.50	3.50
24	gi\|511007306	Glyceraldehyde-3-phosphate dehydrogenase 2	36,013/5.54	550	10 (5)	35	2.91	1.69
25	gi\|511004614	Enolase	47,164/5.56	385	5 (2)	10	1.53	7.99
26	gi\|511006733	Pyruvate kinase	59,079/6.19	464	8 (3)	18	7.59	6.44
27	gi\|511006733	Pyruvate kinase	59,079/6.19	109	4 (0)	3	4.23	3.97
28	gi\|511004430	Pyruvate dehydrogenase complex	52,323/6.11	170	2 (1)	5	17.82	1.36
29	gi\|511004430	Pyruvate dehydrogenase complex	53,161/5.76	123	2 (1)	5	21.82	4.58
30	gi\|511005313	Pyruvate dehydrogenase E1 component subunit alpha	43,770/8.31	246	6 (2)	11	6.89	1.35
31	gi\|511002644	Pyruvate dehydrogenase E2 component	53,161/5.76	183	4 (1)	8	1.93	1.51
32	gi\|511002644	Pyruvate dehydrogenase E2 component	53,161/5.76	288	4 (2)	8	50.48	21.50
33	gi\|511003312	2,3-Bisphosphoglycerate-independent phosphoglycerate mutase	56,737/5.23	332	8 (3)	16	0.48	0.41
34	gi\|511003312	2,3-Bisphosphoglycerate-independent phosphoglycerate mutase	56,737/5.23	118	3 (0)	5	0.46	0.62
35	gi\|511010411	Glucose-6-phosphate dehydrogenase	58,358/6.72	96	3 (0)	12	1.62	1.51
36	gi\|511005381	Transaldolase	35,798/5.45	137	3 (0)	9	2.64	0.71
37	gi\|511001250	Transketolase	74,273/5.71	108	4 (1)	8	2.64	2.56
38	gi\|511005018	NAD⁺:isocitrate dehydrogenase, mitochondrial	41,006/7.02	735	11 (7)	34	0.53	0.62
39	gi\|511005320	NADP⁺:isocitrate dehydrogenase, mitochondrial	47,644/5.97	164	4 (1)	11	0.31	0.57
40	gi\|511002271	Succinyl-CoA ligase, mitochondrial	47,555/5.89	138	4 (1)	12	0.55	0.39
41	gi\|511009244	Succinate dehydrogenase	71,166/6.15	112	3 (1)	7	0.71	0.55
42	gi\|511006629	Fumarate hydratase, mitochondrial	53,753/6.65	410	4 (4)	11	0.55	0.39
43	gi\|511003684	Aldehyde dehydrogenase	54,639/5.51	368	10 (4)	20	1.52	1.95
44	gi\|511001067	Aldehyde dehydrogenase	53,854/5.57	284	5 (2)	14	1.64	1.72
45	gi\|511008662	Acetyl-CoA C-acetyltransferase	41,434/5.65	507	8 (4)	23	0.39	0.43
46	gi\|511005081	Phosphoglucomutase	61,303/5.61	151	4 (1)	11	3.50	4.55
47	gi\|511006219	UTP-glucose-1-phosphate uridylyltransferase	56,716/6.15	655	10 (3)	23	3.21	4.01
48	gi\|511006325	UDP-glucose 4-epimerase	39,235/5.76	138	3 (0)	12	1.07	1.55
49	gi\|511009038	Galactokinase	48,847/6.00	209	5 (0)	12	2.17	2.26
50	gi\|511010174	S-Formylglutathione hydrolase	32,160/6.11	61	2 (0)	3	2.27	2.68
51	gi\|758354396	Thiazole biosynthetic enzyme	33,882/5.28	345	3 (1)	18	20.96	14.71
Protein metabolism
52	gi\|511000792	40S ribosomal protein S21	9718/4.93	287	3 (2)	66	0.34	0.35
53	gi\|511011908	40S ribosomal protein S7	21,057/9.70	71	1 (1)	12	0.07	0.12
54	gi\|511008091	Eukaryotic translation initiation factor 5A	17,694/5.08	302	3 (3)	19	0.13	0.68
55	gi\|511011759	Elongation factor Tu	51,841/6.26	294	5 (2)	13	0.17	0.09
56	gi\|511006438	Nascent polypeptide-associated complex subunit beta	17,501/5.34	362	5 (3)	48	0.02	0.06
57	gi\|511003551	26S protease regulatory subunit 6A	48,455/4.96	346	9 (2)	18	0.29	0.33
58	gi\|511009637	26S protease regulatory subunit 6B	46,545/5.72	228	4 (2)	15	0.42	0.38
59	gi\|511010098	20S proteasome subunit Alpha 6	31,144/5.06	254	4 (2)	15	0.01	0.02
Nucleic acid metabolism
60	gi\|511005974	Phosphoribosylaminoimidazole-succinocarboxamide synthase	33,792/5.10	231	3 (2)	11	0.49	0.60
61	gi\|511011778	Adenylyl-sulfate kinase	22,883/5.75	387	8 (2)	32	0.20	0.12
62	gi\|511006145	Dihydroorotase, homodimeric type	39,652/5.83	233	5 (0)	13	0.43	0.45
Energy metabolism
63	gi\|511006698	Adenylate kinase 1	28,268/7.01	624	10 (6)	51	1.61	2.53
64	gi\|511006698	Adenylate kinase 1	28,268/7.01	571	8 (5)	29	37.78	20.29
65	gi\|511003961	V-type H+-transporting ATPase subunit I	95,461/5.05	193	3 (1)	5	1.61	1.51
66	gi\|511007631	V-type proton ATPase catalytic subunit A	70,756/5.07	361	6 (3)	15	3.23	1.72
Signal transduction
67	gi\|511007036	14-3-3 family protein epsilon	29,228/4.84	175	5 (1)	24	1.75	2.01
Molecular chaperone
68	gi\|511004981	Heat shock 70 kDa protein 1/8	67,760/5.10	280	3 (2)	7	4.23	3.64
69	gi\|511004335	Hsp70-like protein	71,389/5.79	202	4 (1)	7	2.38	2.99
70	gi\|511003138	Peptidyl-prolyl cis–trans isomerase cyp5	19,078/6.92	477	8 (5)	47	1.72	1.64
Redox homeostasis
71	gi\|511008071	Peroxiredoxin	22,926/4.94	254	3 (2)	25	20.46	2.95
72	gi\|511008071	Peroxiredoxin	22,926/4.94	114	2 (1)	12	15.30	13.16
73	gi\|511008071	Peroxiredoxin	22,926/4.94	173	3 (1)	14	16.70	17.01
74	gi\|503389911	Glutathione peroxidase	20,881/6.40	61	1 (0)	5	1.58	3.42
75	gi\|511008344	Oxidoreductase	30,743/6.25	432	5 (2)	15	1.55	3.44
76	gi\|511009098	Oxidoreductase	28,164/6.54	310	6 (2)	28	1.84	1.66
77	gi\|511009792	Ferritin heavy chain	20,301/5.23	268	5 (3)	39	2.30	2.08
Hypothetical protein
78	gi\|511011920	Hypothetical protein HMPREF1544_00199	91,261/6.42	211	5 (1)	5	23.83	8.50
79	gi\|511010665	Hypothetical protein HMPREF1544_01177	55,006/4.72	85	2 (1)	6	4.84	2.65
80	gi\|511010454	Hypothetical protein HMPREF1544_01386	28,881/6.42	101	2 (0)	6	41.34	58.10
81	gi\|511009952	Hypothetical protein HMPREF1544_01888	14,839/5.89	125	1 (1)	10	4.23	2.59
82	gi\|511009952	Hypothetical protein HMPREF1544_01888	14,839/5.89	293	3 (2)	32	3.18	1.74
83	gi\|511009799	Hypothetical protein HMPREF1544_02101	47,569/7.79	191	3 (2)	10	0.35	0.43
84	gi\|511009692	Hypothetical protein HMPREF1544_02181	22,351/5.33	175	3 (2)	13	23.25	37.32
85	gi\|511009605	Hypothetical protein HMPREF1544_02260	26,248/5.37	175	3 (1)	12	9.70	3.04
86	gi\|511008946	Hypothetical protein HMPREF1544_03035	42,241/7.22	630	8 (4)	26	34.49	15.14
87	gi\|511008765	Hypothetical protein HMPREF1544_03136	53,353/5.77	422	5 (4)	15	0.06	0.07
88	gi\|511008618	Hypothetical protein HMPREF1544_03262	68,758/6.29	378	7 (3)	12	0.01	0.01
89	gi\|511008350	Hypothetical protein HMPREF1544_03549	43,856/7.82	229	4 (2)	9	13.89	17.99
90	gi\|511008286	Hypothetical protein HMPREF1544_03639	48,153/5.50	235	5 (2)	10	0.11	0.25
91	gi\|511008150	Hypothetical protein HMPREF1544_03790	20,026/5.04	307	4 (2)	22	0.16	0.34
92	gi\|511008020	Hypothetical protein HMPREF1544_03919	29,429/6.97	425	6 (4)	37	0.20	0.19
93	gi\|511007940	Hypothetical protein HMPREF1544_03956	29,843/5.14	123	2 (1)	10	25.34	20.07
94	gi\|511007175	Hypothetical protein HMPREF1544_04707	63,905/5.35	106	2 (0)	4	0.09	0.09
95	gi\|511006887	Hypothetical protein HMPREF1544_05024	55,228/6.28	275	3 (2)	9	2.16	5.78
96	gi\|511006887	Hypothetical protein HMPREF1544_05024	55,228/6.28	309	5 (2)	10	5.47	5.35
97	gi\|511006017	Hypothetical protein HMPREF1544_05863	30,010/5.58	423	4 (4)	23	2.45	2.18
98	gi\|511004978	Hypothetical protein HMPREF1544_06871	18,967/4.96	249	3 (2)	13	5.27	17.90
99	gi\|511004902	Hypothetical protein HMPREF1544_06986	28,947/9.05	103	1 (1)	6	3.61	2.41
100	gi\|511004522	Hypothetical protein HMPREF1544_07306	26,114/5.56	252	4 (2)	21	7.99	3.58
101	gi\|511004398	Hypothetical protein HMPREF1544_07438	21,313/5.70	199	3 (1)	13	0.29	0.18
102	gi\|511004099	Hypothetical protein HMPREF1544_07752	21,249/5.14	294	6 (2)	31	24.54	3.86
103	gi\|511003967	Hypothetical protein HMPREF1544_07893	16,356/4.59	152	3 (1)	30	0.04	0.20
104	gi\|511003188	Hypothetical protein HMPREF1544_08616	30,249/5.39	224	4 (1)	11	28.24	26.23
105	gi\|511003039	Hypothetical protein HMPREF1544_08758	19,098/8.39	392	4 (3)	25	4.40	2.25
106	gi\|511003039	Hypothetical protein HMPREF1544_08758	19,098/8.39	347	8 (2)	70	8.61	9.91
107	gi\|511002615	Hypothetical protein HMPREF1544_09175	26,203/5.36	170	4 (1)	22	6.71	3.31
108	gi\|511002606	Hypothetical protein HMPREF1544_09235	58,903/4.89	98	1 (1)	5	23.78	30.46
109	gi\|511002606	Hypothetical protein HMPREF1544_09235	58,903/4.89	81	2 (0)	5	5.99	20.72
110	gi\|511001781	Hypothetical protein HMPREF1544_10038	43,811/6.02	96	1 (1)	4	8.25	14.78
111	gi\|511001506	Hypothetical protein HMPREF1544_10287	14,404/4.95	156	3 (0)	26	24.12	22.30
112	gi\|511001144	Hypothetical protein HMPREF1544_10622	24,389/5.25	162	3 (1)	15	5.08	4.08
113	gi\|511001065	Hypothetical protein HMPREF1544_10686	38,562/6.58	161	5 (0)	20	26.39	17.31
114	gi\|511000884	Hypothetical protein HMPREF1544_10852	100,320/8.41	161	3 (1)	5	0.04	0.00
115	gi\|511000884	Hypothetical protein HMPREF1544_10852	100,320/8.41	484	6 (4)	10	0.04	0.01
116	gi\|511000884	Hypothetical protein HMPREF1544_10852	10,0320/8.41	210	4 (1)	7	0.01	0.02
117	gi\|511000397	Hypothetical protein HMPREF1544_11327	66,634/5.41	127	3 (0)	8	24.47	16.26
118	gi\|511000331	Hypothetical protein HMPREF1544_11393	14,081/6.75	138	3 (1)	38	2.61	3.05

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