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Table 1 Plasmids used in this work

From: New approaches to achieve high level enzyme production in Streptomyces lividans

Plasmid

Characteristics

Reference

pN702GEM3

E. coliStreptomyces shuttle vector; contains the pIJ702 origin of replication (for Streptomyces), the pUC origin of replication (for E. coli) and a polylinker derived from pGEM3Zf(+) (Promega). It carries the bifunctional Neo/Kan resistance marker from Tn5 (for both E. coli and Streptomyces)

[21]

pNX30

pN702GEM3 derivative. xysA xylanase ORF without any promoter and flanked by transcriptional terminators. Used as negative control

[35]

pNX24

pN702GEM3 derivative. xysA promoter from S. halstedii controls xylanase expression

[22]

pNUF5

pN702GEM3 derivative. pstS promoter from S. lividans controls xylanase expression

[20]

pNErmX

pN702GEM3 derivative. Erythromycin resistance promoter from S. erythraeus controls xylanase expression

This work

pNvsi

pN702GEM3 derivative. vsi promoter from S. venezuelae controls xylanase expression

This work

pNX26

pN702GEM3 derivative. Xylose isomerase promoter from S. coelicolor controls xylanase expression

This work

pNXHid

pN702GEM3 derivate. Promoter of a putative hydrolase (glpQ) from S. coelicolor controls xylanase expression

This work

pNXA1

pNX24 derivate. xysA promoter from S. halstedii controls xylanase expression with amylase signal peptide

This work

pNXA2

pNX24 derivative. xysA promoter from S. halstedii. Controls xylanase expression with the amylase signal peptide with three additional amino acids

This work

pNXAmy

pN702GEM3 derivative. xysA promoter from S. halstedii controls amylase expression

This work

pNUFAmy

pN702GEM3 derivative. pstS promoter from S. lividans controls amylase expression

This work

pEPOS101

pHJL401 derivative harbouring xysAp driving the expression of SLAC (SCO6712)

This work