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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Transcriptome-enabled discovery and functional characterization of enzymes related to (2S)-pinocembrin biosynthesis from Ornithogalum caudatum and their application for metabolic engineering

Fig. 3

HPLC analysis of reaction products from E. coli [pET28a] (1), heat denatured protein extracts derived from E. coli [pET28a-Oc4CL1] (2) and crude protein extracts of E. coli [pET28a-Oc4CL1] (3) using trans-cinnamic acid (a), ρ-coumaric acid (b), caffeic acid (c), and ferulic acid (d) as the substrates. R2, X2, C2, A2, R1, X1, C1 and A1 refer to substrates trans-cinnamic acid (5), ρ-coumaric acid (6), caffeic acid (7), ferulic acid (8), and their corresponding products trans-cinnamoyl CoA (10), 4-coumaroyl CoA (11), caffeoyl CoA (12) and feruloyl CoA (13), respectively. Detection was set at 270 nm for reaction product of trans-cinnamic acid (5), 320 nm for enzymatic products of ρ-coumaric acid (6), caffeic acid (7) and ferulic acid (8). mAU Milliabsorbance units

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Microbial Cell Factories

ISSN: 1475-2859

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