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Table 5 The glycerol dehydrogenase and glycerol kinase activity (mU/mg protein) of C. werkmaniidhaDldhAadhE::ChlFRT

From: High yield 1,3-propanediol production by rational engineering of the 3-hydroxypropionaldehyde bottleneck in Citrobacter werkmanii

Carbon source

Enzyme activity (mU/mg protein)

Glycerol dehydrogenase

Glycerol kinase

Glycerol

72.70 ± 1.76

10.67 ± 1.34

Glycerol + Glucose

26.80 ± 0.18

13.85 ± 0.20

  1. The strain was grown under anaerobic conditions in fermentation medium with glycerol only or with 0.33 molar ratio glucose to glycerol in batch cultivations on bioreactor scale. The values are the averages of two experiments with their corresponding standard deviations