High yield 1,3-propanediol production by rational engineering of the 3-hydroxypropionaldehyde bottleneck in Citrobacter werkmanii

Table 2 Growth rate, PDO yield, NAD⁺/NADH-ratio and pH of C. werkmanii knock-outs

Strains	Growth rate (h⁻¹)	PDO Yield (mol/mol)	NAD⁺/NADH ratio	Final pH
∆dhaD	0.25 ± 0.01	0.68 ± 0.05	4.74 ± 0.14	6.43 ± 0.01
∆dhaD∆ldhA	0.31 ± 0.03	0.84 ± 0.01	1.66 ± 0.03	6.35 ± 0.02
∆dhaD∆adhE::ChlFRT	0.34 ± 0.01	0.96 ± 0.01	2.20 ± 0.07	6.33 ± 0.03
∆dhaD∆ldhA∆adhE::ChlFRT	0.13 ± 0.01	1.00 ± 0.03	2.38 ± 0.15	6.80 ± 0.00

C. werkmanii ∆dhaD, C. werkmanii ∆dhaD∆ldhA, C. werkmanii ∆dhaD∆adhE::ChlFRT and C. werkmanii ∆dhaD∆ldhA∆adhE::ChlFRT grown anaerobically in shake flasks. The values are the average of two experiments with their corresponding standard deviation

ISSN: 1475-2859