Adaptive evolution and metabolic engineering of a cellobiose- and xylose- negative Corynebacterium glutamicum that co-utilizes cellobiose and xylose

Table 3 Fatty acid profiles of the cellobiose-utilizing C. glutamicum strains and the wild-type

Fatty acid composition (%)	Cg-pBbEB1c	Cg-Cello01(evo)	Cg-Cello02(evo)
C_10:0	0.15	0.08	0.08
C_12:0	0.25	–	–
C_12:0 3OH	–	0.06	0.05
C_14:0	1.33	0.43	0.39
C_16:1 w9c	0.84	0.51	0.46
C_16:0	41.04	35.89	33.28
C_16:0 3OH	0.31	0.69	0.34
C_18:1 w9c	54.24	59.97	63.4
C_18:0	0.47	0.44	0.41
C_18:0 10-methyl	1.27	1.54	1.26
C_19:1 iso I	0.09	0.33	0.33
C_19:1 w6c/unknown fatty acids*	–	0.06	–

The Cg-Cello01(evo) and Cg-Cello02(evo) strains were cultivated with 2 % cellobiose as sole carbon source. The Cg-pBbEB1c stain was cultivated with 2 % glucose as sole carbon source. The fatty acid profiles of the Cg-pBbEB1c were almost identical to the profiles of the C. glutamicum wild-type [23]. Analysis of fatty acid and fatty acid methyl ester were followed by the standard protocol of Sherlock^® Microbial Identification System (MIS) of MIcrobial IDentification Inc. (MIDI)
Data represents mean values of duplicated cultivations
* Equivalent chain lengths (ECL) value = 18.846

ISSN: 1475-2859