Adaptive evolution and metabolic engineering of a cellobiose- and xylose- negative Corynebacterium glutamicum that co-utilizes cellobiose and xylose

Table 2 List of common mutations of C. glutamicum Cg-Cello01(evo) and Cg-Cello02(evo) strains

Reference position	Gene name	Type	Reference nucleotide	Allele nucleotide	Coding region change	Amino acid change	Annotation
32227	cg0045	MISSENSE	A	T	551A > T	Asn184Ile	Probable ABC transport protein, membrane component
364912	cg0414	MISSENSE	A	C	1089A > C	Glu363Asp	Wzz, cell surface polysaccharide biosynthesis/chain length determinant protein
1689677	cg1796	MISSENSE	C	T	103G > A	Glu35Lys	RibX, putative membrane protein-C. ammoniagenes RibX homolog
2041951	cg2118 ^a	MISSENSE	G	T	224G > T	Gly75Val	FruR, transcriptional regulator of sugar metabolism, DeoR family
2058943	cg2135	MISSENSE	A	G	490T > C	Ser164Pro	MiaB, tRNA methylthiotransferase
2296630	cg2380	Deletion	C	–	270delG	Gly90 fs	Hypothetical protein Cg2380
2331324	cg2412	MISSENSE	C	T	154C > T	Pro52Ser	Hypothetical protein Cg2412
2545730	cg2637 ^b	MISSENSE	G	A	436G > A	Asp146Asn	BenA, benzoate 1,2-dioxygenase alpha subunit (aromatic ring hydroxylation dioxygenase A)
2607484	cg2705	MISSENSE	G	A	407C > T	Ala136Val	AmyE, maltose-binding protein precursor
2826260	cg2941	MISSENSE	T	C	577A > G	Ile193Val	LysE type translocator

Full list of all mutations of C. glutamicum Cg-Cello01(evo) and Cg-Cello01(evo) strains were described in the Additional file 1: Table S1 and S2
^aThe mRNA expression of the cg2118 gene was highly up-regulated
^bThe mRNA expression of the cg2637 gene was highly down-regulated. See the details in the text

ISSN: 1475-2859