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Fig. 7 | Microbial Cell Factories

Fig. 7

From: Adaptive evolution and metabolic engineering of a cellobiose- and xylose- negative Corynebacterium glutamicum that co-utilizes cellobiose and xylose

Fig. 7

Additional sugar transporters for co-consumption of cellobiose and xylose. Two different cellobiose-positive strains [Cg-Cello03-Xyl01 (top left panel) and Cg-Cello04-Xyl01 (top right panel)] co-expressing XylA and XylB were further engineered with additional sugar transporters such as a Gxf1 (Candia intermedia) [29] and Sut1 (Pichia stipites) [30], yielding Cg-Cello03-Xyl02 and Cg-Cello03-Xyl03 (left panels) and Cg-Cello04-Xyl02 and Cg-Cello04-Xyl03 (right panels), respectively. The cellobiose-positive and xylose-positive strains were cultivated in CgXII medium with a mixture of 2 % (w/v) cellobiose and 1 % (w/v) xylose. Cellobiose (blue square), xylose (red circle) were shown. Data represents mean values of at least three cultivations and error bars represent standard deviations

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Microbial Cell Factories

ISSN: 1475-2859

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