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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Adaptive evolution and metabolic engineering of a cellobiose- and xylose- negative Corynebacterium glutamicum that co-utilizes cellobiose and xylose

Fig. 5

A comparison of cell growth and cellobiose consumption of evolved C. glutamicum strains. The evolved C. glutamicum strains was compared with reconstructed cellobiose-positive chassis of C. glutamicum strains. By plasmid-curing and re-transformation of pBbEB1c-bG plasmid, Cg-Cello03 (upper panels; open symbols) and Cg-Cello04 (lower panels; open symbols) strains as reconstructed cellobiose-positive chassis were obtained from Cg-Cello01(evo) (upper panels; solid symbols) and Cg-Cello02(evo) (lower panels; solid symbols) strains, respectively. The strains were cultivated in CgXII medium with 2 % (w/v) cellobiose as sole carbon source after the pre-cultivation in BHIS medium. Growth (left panels; black circle), cellobiose (right panels; blue square), glucose (right panels; red triangle) were shown. Data represents mean values of at least three cultivations

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