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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Adaptive evolution and metabolic engineering of a cellobiose- and xylose- negative Corynebacterium glutamicum that co-utilizes cellobiose and xylose

Fig. 3

Measurement of β-glucosidase (bG) and glucokinase (Glk) activities in C. glutamicum wild-type or evolved strains. The Cg-pBbEB1c (black) and Cg-Cello02 (grey) were cultivated in CgXII medium with 2 % (w/v) glucose. The evolved Cg-Cello01(evo) (red) and Cg-Cello02(evo) (blue) strains were cultivated in CgXII medium with 2 % (w/v) cellobiose. The cell extract (E) and cell-free supernatant (S) were used for the measurement of the bG activities (U/mg protein) when the strains were cultivated for 24 h (upper panel). The cell extracts from the strains grown for 6, 12, or 24 h were used for the measurement of the Glk activities (mU/mg protein) (lower panel). Data represents mean values of at least three cultivations and error bars represent standard deviations. (N.D.) not detected

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Microbial Cell Factories

ISSN: 1475-2859

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