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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Adaptive evolution and metabolic engineering of a cellobiose- and xylose- negative Corynebacterium glutamicum that co-utilizes cellobiose and xylose

Fig. 2

Adaptive evolution of engineered C. glutamicum strains. a Scheme of metabolic engineering and adaptive evolution of Cg-Cello01 and Cg-Cello02 strains were described. CoryneBrick vectors containing the cdt-1 and/or gh1-1 gene(s) were introduced into C. glutamicum wild-type, in which no growth and consumption of cellobiose were observed (b and c; the first column). Growth of Cg-Cello01 and Cg-Cello02 strain was not appeared initially. However, the maximal cell growths of Cg-Cello01 and Cg-Cello02 were observed after 16 d (b; the second column) and after 11 d (c; the second column), respectively. Subsequently, serial cell transfers were performed for adaptive evolutions of Cg-Cello01 and Cg-Cello02 in 48 h (b and c). Finally, Cg-Cello01(evo) and Cg-Cello02(evo) were obtained since growth and cellobiose consumption were unchanged. Growth at OD600, cellobiose (g/L) and glucose (g/L) were shown in a symbol of circle (black), square (blue) and triangle (red), respectively. Data represents mean values of at least three cultivations. (N.A.) not available

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