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Table 2 Yields of antibody fragments purified from shake flask cultures

From: Systematic screening of soluble expression of antibody fragments in the cytoplasm of E. coli

Fragment type

Antibody name

Purified yield

scFv

Herceptin (human IgG1)

251 mg/L (with CyDisCo)

167 mg/L (without CyDisCo)

Tysabri (human IgG4)

169 mg/L (with CyDisCo)

141 mg/L (without CyDisCo)

3211 (mouse IgG1)

11 mg/L

3M8O (human IgA1)

68 mg/L

2R56 (human IgE)

32 mg/L

Fab

Maa48 (human IgG1)

42 mg/L

3211 (mouse IgG1)

13 mg/L

3M8O (human IgA1)

19 mg/L

1QLR (human IgM)

11 mg/L

  1. Yields were determined by A280 measurements. Extinction coefficients used were calculated using ProtParam and assuming all of the cysteines present are in disulfide bonds. Except where stated all fragments were made in the presence of CyDisCo components