Fig. 4
From: Systematic screening of soluble expression of antibody fragments in the cytoplasm of E. coli
Structure of the expression vectors used in this study. a scFv vector. The spacer region including the KasI and XhoI sites encodes for the sequence -Gly-Ala-Ser-(Gly4-Ser)3-Ser- while the hexahistidine-tag including the BamHI site adds Gly-Ser-His6. rbs = ribsome binding site. The initiating Met is included in the NdeI site (CATATG); b Fab vector. This polycistronic vector includes two ribosome binding sites (rbs) to initiate translation of the heavy and light chain. The hexahistdine-tag including the BamHI site adds Gly-Ser-His6