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Table 1 Bacterial strains and plasmids used in this study

From: Metabolic engineering of Escherichia coli for the production of cinnamaldehyde

  Description Reference or source
Strain
 XL1-Blue recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [FproAB lacI q Z ΔM15 Tn10 (Tet r)] Stratagene1
 BL21(DE3) E. coli B F dcm ompT hsdS(r B m B ) gal λ(DE3) Novagen2
 W3110 F λ rph-1 INV(rrnD, rrnE) CGSC no. 44743
 YHP01 W3110 Δcrr This study
 YHP02 W3110 Δcrr ΔtyrR This study
 YHP03 W3110 Δcrr ΔtyrR ΔtrpE This study
 YHP04 W3110 Δcrr ΔtyrR ΔtrpE ΔtyrA This study
 YHP05 W3110 Δcrr ΔtyrR ΔtrpE ΔtyrA ΔpykA This study
Plasmid
 pTac15k KmR, p15A origin, tac promoter, 4.0 kb [54]
 pTrc99A AmpR, pBR322 origin, trc promoter, 4.2 kb Pharmacia4
 pUC19 AmpR, pMB1 origin, lac promoter, 2.6 kb New England biolabs5
 pET-22b AmpR, pBR322 origin, T7 promoter, 5.5 kb EMD chemicals6
 pHB-I01 pET-22b derivatives, PT7AtPAL1 (N-term 8× His-tag) This study
 pHB-I02 pET-22b derivatives, PT7At4CL1 (N-term 8× His-tag) This study
 pHB-I03 pET-22b derivatives, PT7AtCCR (N-term 8× His-tag) This study
 pHB-I04 pET-22b derivatives, PT7SmPAL (N-term 8× His-tag) This study
 pHB-I05 pET-22b derivatives, PT7ScCCL (N-term 8× His-tag) This study
 pHB-CA pTrc99A derivatives, PtrcSmPAL (C-term FLAG-tag) This study
 pHB-CAD pTrc99A derivatives, PtrcSmPAL (C-term FLAG-tag)–ScCCL (N-term 8× His-tag)–AtCCR (N-term 8× His-tag) This study
 pUC19G pUC19, aroG8/15 This study
 pUC19B pUC19, ydiB This study
 pUC19K pUC19, aroK This study
 pUC19A pUC19, pheA fbr, dm This study
 pTac15kG pTac15k, PtacaroG8/15 This study
 pTac15kGB pTac15k, PtacaroG8/15ydiB This study
 pTac15kGBK pTac15k, PtacaroG8/15ydiBaroK This study
 pTac15kGBKA pTac15k, PtacaroG8/15ydiBaroKpheA fbr, dm This study
 pYH pTac15k, PtacaroG8/15ydiBaroKpheA fbr, dm, SacII region modification This study
 pPpc113 pUC19, synthetic BBa_J23113 Anderson promoter (Ppc113) This study
 pTrc99A-mod pTrc99A, MCS modification (SpeI, NotI) Lab stock
 pGlk pTrc99A-mod derivatives, Ppc113–glk–Tlpp This study
 pGalP pTrc99A-mod derivatives, Ppc113–galP–Tlpp This study
 pYH-glk pYH derivatives, Ppc113–glk–Tlpp This study
 pYHP pYH-glk derivatives, Ppc113–galP–Tlpp This study
 pCW611 AmpR, λ-red recombinase expression plasmid under ara-inducible BAD promoter, cre-loxP recombinase system under lacUV5 promoter, temp sensitive origin [50]
 pEcmuloxC AmpR, CmR, pUG6 derivatives, lox66-cat-lox71 [55]
  1. 1Stratagene cloning systems, La Jolla, CA, USA
  2. 2Novagen, inc., Madison, WI, USA
  3. 3The coli genetic stock center, Yale University, USA
  4. 4Pharmacia biotech, Uppsala, Sweden
  5. 5New England biolabs, inc., Beverly, MA, USA
  6. 6EMD chemicals, Gibbstown, NJ, USA