Table 1 Bacterial strains and plasmids used in this study
From: Metabolic engineering of Escherichia coli for the production of cinnamaldehyde
| Â | Description | Reference or source |
|---|---|---|
Strain | ||
 XL1-Blue | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lacI q Z ΔM15 Tn10 (Tet r)] | Stratagene1 |
 BL21(DE3) | E. coli B F − dcm ompT hsdS(r − B m − B ) gal λ(DE3) | Novagen2 |
 W3110 | F − λ − rph-1 INV(rrnD, rrnE) | CGSC no. 44743 |
 YHP01 | W3110 Δcrr | This study |
 YHP02 | W3110 Δcrr ΔtyrR | This study |
 YHP03 | W3110 Δcrr ΔtyrR ΔtrpE | This study |
 YHP04 | W3110 Δcrr ΔtyrR ΔtrpE ΔtyrA | This study |
 YHP05 | W3110 Δcrr ΔtyrR ΔtrpE ΔtyrA ΔpykA | This study |
Plasmid | ||
 pTac15k | KmR, p15A origin, tac promoter, 4.0 kb | [54] |
 pTrc99A | AmpR, pBR322 origin, trc promoter, 4.2 kb | Pharmacia4 |
 pUC19 | AmpR, pMB1 origin, lac promoter, 2.6 kb | New England biolabs5 |
 pET-22b | AmpR, pBR322 origin, T7 promoter, 5.5 kb | EMD chemicals6 |
 pHB-I01 | pET-22b derivatives, PT7–AtPAL1 (N-term 8× His-tag) | This study |
 pHB-I02 | pET-22b derivatives, PT7–At4CL1 (N-term 8× His-tag) | This study |
 pHB-I03 | pET-22b derivatives, PT7–AtCCR (N-term 8× His-tag) | This study |
 pHB-I04 | pET-22b derivatives, PT7–SmPAL (N-term 8× His-tag) | This study |
 pHB-I05 | pET-22b derivatives, PT7–ScCCL (N-term 8× His-tag) | This study |
 pHB-CA | pTrc99A derivatives, Ptrc–SmPAL (C-term FLAG-tag) | This study |
 pHB-CAD | pTrc99A derivatives, Ptrc–SmPAL (C-term FLAG-tag)–ScCCL (N-term 8× His-tag)–AtCCR (N-term 8× His-tag) | This study |
 pUC19G | pUC19, aroG8/15 | This study |
 pUC19B | pUC19, ydiB | This study |
 pUC19K | pUC19, aroK | This study |
 pUC19A | pUC19, pheA fbr, dm | This study |
 pTac15kG | pTac15k, Ptac–aroG8/15 | This study |
 pTac15kGB | pTac15k, Ptac–aroG8/15–ydiB | This study |
 pTac15kGBK | pTac15k, Ptac–aroG8/15–ydiB–aroK | This study |
 pTac15kGBKA | pTac15k, Ptac–aroG8/15–ydiB–aroK–pheA fbr, dm | This study |
 pYH | pTac15k, Ptac–aroG8/15–ydiB–aroK–pheA fbr, dm, SacII region modification | This study |
 pPpc113 | pUC19, synthetic BBa_J23113 Anderson promoter (Ppc113) | This study |
 pTrc99A-mod | pTrc99A, MCS modification (SpeI, NotI) | Lab stock |
 pGlk | pTrc99A-mod derivatives, Ppc113–glk–Tlpp | This study |
 pGalP | pTrc99A-mod derivatives, Ppc113–galP–Tlpp | This study |
 pYH-glk | pYH derivatives, Ppc113–glk–Tlpp | This study |
 pYHP | pYH-glk derivatives, Ppc113–galP–Tlpp | This study |
 pCW611 | AmpR, λ-red recombinase expression plasmid under ara-inducible BAD promoter, cre-loxP recombinase system under lacUV5 promoter, temp sensitive origin | [50] |
 pEcmuloxC | AmpR, CmR, pUG6 derivatives, lox66-cat-lox71 | [55] |