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Table 1 Bacterial strains and plasmids used in this study

From: Metabolic engineering of Escherichia coli for the production of cinnamaldehyde

 

Description

Reference or source

Strain

 XL1-Blue

recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [FproAB lacI q Z ΔM15 Tn10 (Tet r)]

Stratagene1

 BL21(DE3)

E. coli B F dcm ompT hsdS(r B m B ) gal λ(DE3)

Novagen2

 W3110

F λ rph-1 INV(rrnD, rrnE)

CGSC no. 44743

 YHP01

W3110 Δcrr

This study

 YHP02

W3110 Δcrr ΔtyrR

This study

 YHP03

W3110 Δcrr ΔtyrR ΔtrpE

This study

 YHP04

W3110 Δcrr ΔtyrR ΔtrpE ΔtyrA

This study

 YHP05

W3110 Δcrr ΔtyrR ΔtrpE ΔtyrA ΔpykA

This study

Plasmid

 pTac15k

KmR, p15A origin, tac promoter, 4.0 kb

[54]

 pTrc99A

AmpR, pBR322 origin, trc promoter, 4.2 kb

Pharmacia4

 pUC19

AmpR, pMB1 origin, lac promoter, 2.6 kb

New England biolabs5

 pET-22b

AmpR, pBR322 origin, T7 promoter, 5.5 kb

EMD chemicals6

 pHB-I01

pET-22b derivatives, PT7AtPAL1 (N-term 8× His-tag)

This study

 pHB-I02

pET-22b derivatives, PT7At4CL1 (N-term 8× His-tag)

This study

 pHB-I03

pET-22b derivatives, PT7AtCCR (N-term 8× His-tag)

This study

 pHB-I04

pET-22b derivatives, PT7SmPAL (N-term 8× His-tag)

This study

 pHB-I05

pET-22b derivatives, PT7ScCCL (N-term 8× His-tag)

This study

 pHB-CA

pTrc99A derivatives, PtrcSmPAL (C-term FLAG-tag)

This study

 pHB-CAD

pTrc99A derivatives, PtrcSmPAL (C-term FLAG-tag)–ScCCL (N-term 8× His-tag)–AtCCR (N-term 8× His-tag)

This study

 pUC19G

pUC19, aroG8/15

This study

 pUC19B

pUC19, ydiB

This study

 pUC19K

pUC19, aroK

This study

 pUC19A

pUC19, pheA fbr, dm

This study

 pTac15kG

pTac15k, PtacaroG8/15

This study

 pTac15kGB

pTac15k, PtacaroG8/15ydiB

This study

 pTac15kGBK

pTac15k, PtacaroG8/15ydiBaroK

This study

 pTac15kGBKA

pTac15k, PtacaroG8/15ydiBaroKpheA fbr, dm

This study

 pYH

pTac15k, PtacaroG8/15ydiBaroKpheA fbr, dm, SacII region modification

This study

 pPpc113

pUC19, synthetic BBa_J23113 Anderson promoter (Ppc113)

This study

 pTrc99A-mod

pTrc99A, MCS modification (SpeI, NotI)

Lab stock

 pGlk

pTrc99A-mod derivatives, Ppc113–glk–Tlpp

This study

 pGalP

pTrc99A-mod derivatives, Ppc113–galP–Tlpp

This study

 pYH-glk

pYH derivatives, Ppc113–glk–Tlpp

This study

 pYHP

pYH-glk derivatives, Ppc113–galP–Tlpp

This study

 pCW611

AmpR, λ-red recombinase expression plasmid under ara-inducible BAD promoter, cre-loxP recombinase system under lacUV5 promoter, temp sensitive origin

[50]

 pEcmuloxC

AmpR, CmR, pUG6 derivatives, lox66-cat-lox71

[55]

  1. 1Stratagene cloning systems, La Jolla, CA, USA
  2. 2Novagen, inc., Madison, WI, USA
  3. 3The coli genetic stock center, Yale University, USA
  4. 4Pharmacia biotech, Uppsala, Sweden
  5. 5New England biolabs, inc., Beverly, MA, USA
  6. 6EMD chemicals, Gibbstown, NJ, USA