Table 1 Assay of enzyme activities of crude PhbC, PhbA, PhbB and PpnK in C. crenatum SYPA 5, P1 (SYPA 5/phbCAB) and P2 (SYPA 5/phbCAB-ppnK)
Strains | Specific enzyme activities | ||||
|---|---|---|---|---|---|
PhbC (U/mg) | PhbA (U/mg) | PhbB (U/mg) | ATP-NAD kinase (U/g) | PolyP-NAD kinase (U/g) | |
C. crenatum SYPA 5 | 0.02 ± 0.00 | 0.02 ± 0.00 | 0.06 ± 0.01 | 0.63 ± 0.03 | 0.18 ± 0.01 |
C. crenatum P1 | 0.27 ± 0.01 | 0.58 ± 0.02 | 0.64 ± 0.02 | 0.67 ± 0.04 | 0.20 ± 0.01 |
C. crenatum P2 | 0.29 ± 0.01 | 0.61 ± 0.03 | 0.83 ± 0.03 | 84.35 ± 0.41 | 3.12 ± 0.02 |