Fig. 5
Optimization of MVA pathway by homolog substitution for the lower portion genes. a Expression constructs of the homolog substituted MvL portions. The homolog genes are from S. aureus and represented with prefixion of “Sa” to the gene name. b and c Effect of combinations of MvUM,H and MvL7–13 on protoilludene production and mevalonate accumulation. The culture were carried out in 4 mL of 2YT medium containing 2 % (v/v) glycerol with overlay of 1 mL decane at 30 °C for 48 h