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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Combinatorial engineering of hybrid mevalonate pathways in Escherichia coli for protoilludene production

Fig. 4

Coordination of the lower (MvL) and upper (MvU) portions of MVA pathway for protoilludene production. a Expression constructs of the MvU portions with alternations of promoter and copy-number. MvU operon consists of MvaE and MvaS from E. faecalis. The two genes are cloned into pBBR1MCS-2, pSTV28 and pTrc99A vectors, which are designated as pBMvUL, pSMvUM and pTMvUH, respectively. Ef, the gene from E. faecalis; Plac and Ptrc, lac promoter and trc promoter, respectively. Rectangles show replication origin of each plasmid. b Mevalonate production capacity of recombinant E. coli harboring each of pBMvUL, pSMvUM and pTMvUH. The strains were cultured in 2YT medium at 30 °C for 48 h. c and d Effect of combinations of MvUL,M,H and MvL1–6 on protoilludene production and mevalonate accumulation. The culture were carried out in 4 mL of 2YT medium containing 2 % (v/v) glycerol with overlay of 1 mL decane at 30 °C for 48 h

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