Fig. 3

Optimization of the lower (MvL) portion of MVA pathway by sequential order permutation. a Expression constructs of the sequential order permutated MvL portions. MvL operon, containing MvaK1, MvaK2 and MvaD from S. pneumoniae, and IDI from E. coli, is cloned into pSTV28 vector. Sn, the gene from S. pneumoniae; Ec, the gene from E. coli; Plac, lac promoter. b Effect of sequential order permutated MvLs on protoilludene production and cell growth. The strains were grown for 48 h at 30 °C in 4 mL of 2YT medium containing 2 % (v/v) glycerol and 4 mM mevalonate with overlay of 1 mL decane. The dark and light gray bars indicate protoilludene production and cell growth, respectively