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Table 1 Lactate and byproducts accumulations in the wild-type and engineered strains under aerobic and anaerobic conditions

From: Efficient fermentative production of polymer-grade d-lactate by an engineered alkaliphilic Bacillus sp. strain under non-sterile conditions

Strain

Pyruvate (g/l)

Succinate (g/l)

Lactate (g/l)

Formate (g/l)

Acetate (g/l)

Aerobic

     

 WT

0.27 ± 0.05

0.54 ± 0.13

5.39 ± 0.11a

0.37 ± 0.11

4.78 ± 0.84

 ∆ldh

5.39 ± 0.19

3.63 ± 0.88

0.24 ± 0.06a

0.92 ± 0.15

5.51 ± 1.21

 ∆ldh-pDlac

0.26 ± 0.09

0.32 ± 0.10

6.32 ± 0.19b

0.16 ± 0.08

2.95 ± 0.18

Anaerobic

     

 WT

0.22 ± 0.11

0.50 ± 0.17

5.28 ± 0.10a

1.14 ± 0.19

1.34 ± 0.06

 ∆ldh

4.35 ± 0.04

1.09 ± 0.50

0.10 ± 0.00a

2.86 ± 0.25

2.07 ± 0.84

 ∆ldh-pDlac

0.18 ± 0.06

0.53 ± 0.18

6.95 ± 0.02b

0.54 ± 0.11

1.17 ± 0.32

  1. WT, Bacillus sp. N16-5 wild-type strain; ∆ldh, Bacillus sp. N16-5∆ldh strain; ∆ldh-pDlac, Bacillus sp. N16-5∆ldh strain carrying pDlac expression vector
  2. Values were represented as the average ± standard deviation of three independent experiments
  3. a l-lactate product
  4. b d-lactate product