Markedly improving asymmetric oxidation of 1-(4-methoxyphenyl) ethanol with Acetobacter sp. CCTCC M209061 cells by adding deep eutectic solvent in a two-phase system

Table 5 Preparative scale oxidation of racemic MOPE catalyzed by Acetobacter sp. CCTCC M209061 cells in various reaction systems

Reaction media	Optimal substrate concentration (mmol/L)	Initial rate (μmol/min)	Reaction time (h)	Conversion^a (%)	e.e. ^b (%)
Buffer	30.0	95.0	9.0	45.6	85.0
C₄MIM·PF₆/buffer	65.0	90.3	10.0	49.2	97.9
C₄MIM·PF₆/[ChCl][Gly]-containing buffer	80.0	113.3	7.0	50.2	99.9

Reaction conditions: (1) aqueous buffer system: 500 mL TEA-HCl buffer (100 mmol/L, pH 6.5); 30 mmol/L MOPE, 0.3 g/mL immobilized beads; 70 mmol/L acetone; 30 °C; 180 rpm. (2) [C₄MIM][PF₆]/buffer system: 400 mL TEA-HCl buffer (100 mmol/L, pH 6.5), 100 mL [C₄MIM][PF₆]; 65 mmol/L MOPE; 0.3 g/mL immobilized beads; 122 mmol/L acetone; 30 °C; 220 rpm. (3) [C₄MIM][PF₆]/[ChCl][Gly]-containing buffer system: 360 mL TEA-HCl buffer (100 mmol/L, pH 6.5); 100 mL [C₄MIM][PF₆]; 40 mL [ChCl][Gly]; 80 mmol/L MOPE; 0.3 g/mL immobilized beads; 122 mmol/L acetone; 30 °C; 220 rpm
^aThe maximum conversion
^bThe residual substrate e.e.

ISSN: 1475-2859