Fig. 5
Operational stability of immobilized Acetobaceter sp. CCTCC M209061 cells in various reaction systems. Reaction conditions: (1) aqueous buffer system: 500 mL TEA-HCl buffer (100 mmol/L, pH 6.5); 30 mmol/L MOPE; 0.3 g/mL immobilized beads; 70 mmol/L acetone; 30 °C; 180 rpm. (2) [C4MIM][PF6]/buffer biphasic system: 400 mL TEA-HCl buffer (100 mmol/L, pH 6.5), 100 mL [C4MIM][PF6]; 65 mmol/L MOPE; 0.3 g/mL immobilized beads; 122 mmol/L acetone; 30 °C; 220 rpm. (3) [C4MIM][PF6]/[ChCl][Gly]-containing buffer biphasic system:360 mL TEA-HCl buffer (100 mmol/L, pH 6.5); 100 mL [C4MIM][PF6]; 40 mL [ChCl][Gly]; 80 mmol/L MOPE; 0.3 g/mL immobilized beads; 122 mmol/L acetone; 30 °C; 220 rpm