Table 3 Specific activity and protein concentration of the WT and variants
Variants | Protein concentrationa (μg/mL) | Specific activity (U/mg) |
|---|---|---|
M1 (ΔCBM41) | 72.5 ± 2.2 | 1167.3 ± 35.0 |
M3 (ΔX25) | 31.7 ± 0.7 | 1124.4 ± 24.7 |
M5 (ΔCBM41ΔX25) | 55.9 ± 1.8 | 1271.1 ± 41.9 |
Wild-type | 26.6 ± 0.5 | 1096.5 ± 21.9 |