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Table 2 Pullulanase activity of the WT and variants

From: Downsizing a pullulanase to a small molecule with improved soluble expression and secretion efficiency in Escherichia coli

Variants

IA (U/mL)

TA (U/mL)

M1 (ΔCBM41)

84.6 ± 2.6

84.6 ± 2.6

M2 (ΔX45)

M3 (ΔX25)

35.7 ± 1.1

35.7 ± 1.1

M4 (Δ45ΔX25)

M5 (ΔCBM41ΔX25)

71.0 ± 2.4

71.0 ± 2.4

M6 (ΔCBM41ΔX45)

Wild-type

29.1 ± 0.5

29.1 ± 0.5

  1. IA intracellular activity; TA total activity
  2. – Below the detection line