Table 1 List of nucleotide sequences used in this study
Primer name | Nucleotide sequence (5′ → 3′) | Enzyme | Gene |
|---|---|---|---|
WT[F] | GGGAATTCCATATGGATTCTACTTCGACTAAAGTTATTGTTC | NdeI | BaPul |
M1[F] | GGGAATTCCATATGCCATCTGTTTCAAATGCCTATCTTG | NdeI | BaPul-ΔCMB41 |
M2[F] | GGGAATTCCATATGGATTCTACTTCGACTAAAGTTATTGTTC | NdeI | BaPul-ΔX45 |
M3[F] | GGGAATTCCATATGGATTCTACTTCGACTAAAGTTATTGTTC | NdeI | BaPul-ΔX25 |
M4[F] | GGGAATTCCATATGGTAACTGCCGTTCTTGTTGGAGATTTAC | NdeI | BaPul-ΔCMB41ΔX45 |
M5[F] | GGGAATTCCATATGCCATCTGTTTCAAATGCCTATCTTG | NdeI | BaPul-ΔCMB41ΔX25 |
M6[F] | GGGAATTCCATATGGATTCTACTTCGACTAAAGTTATTGTTC | NdeI | BaPul-ΔX25ΔX45 |
pelB-M1[F] | CATGCCATGGATCCATCTGTTTCAAATGCCTATCTTG | NcoI | BaPul-ΔCMB41 |
pelB-M3[F] | CATGCCATGGATTCTACTTCGACTAAAGTTATTGTTC | NcoI | BaPul-ΔX25 |
pelB-M5[F] | CATGCCATGGATCCATCTGTTTCAAATGCCTATCTTG | NcoI | BaPul-ΔCMB41ΔX25 |
pelB-WT[F] | CATGCCATGGATTCTACTTCGACTAAAGTTATTGTTC | NcoI | BaPul |
[R]a | CCGCTCGAGTTGTTTGAGAATAAGCGTACTTATAG | XhoI |