Fig. 5From: Downsizing a pullulanase to a small molecule with improved soluble expression and secretion efficiency in Escherichia coli Effect of pH and temperature on the activities of the WT and variants. a pH. Assays were carried out at 60 °C for 10 min in buffers with pH values within the range of 3.0–7.0. b Temperature. Assays were conducted in 100 mM sodium acetate–acetic acid buffer (pH 5.0) within the temperature range of 40–80 °C for 10 minBack to article page