Fig. 2From: Downsizing a pullulanase to a small molecule with improved soluble expression and secretion efficiency in Escherichia coli SDS-PAGE analysis of the WT and variants. Lanes: M, protein molecular mass markers; C control [vector pET28a(+) without foreign gene]; M1; M2; M3; M4; M5; M6; WT. The values are listed as molecular sizes in kilodaltons. a Total cellular protein. b Soluble fraction of total cellular proteinBack to article page