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Table 2 Bacterial strains and plasmids used in this study

From: Enhanced production of recombinant proteins with Corynebacterium glutamicum by deletion of insertion sequences (IS elements)

  Relevant characteristics Reference or source
Strain   
 E. coli   
  XL1-blue recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proABlacIq ZΔM15 Tn10 (Tet r )] Stratagenea
 C. glutamicum   
  ATCC 13032 Biotin-auxotrophic wild type ATCC
  WJ001 ATCC 13032 with in frame deletion of ISCg2 family (ISCg1a) This study
  WJ002 ATCC 13032 with in frame deletion of ISCg2 family (ISCg1a, ISCg1b) This study
  WJ003 ATCC 13032 with in frame deletion of ISCg2 family (ISCg1a, ISCg1b, ISCg1c) This study
  WJ004 ATCC 13032 with in frame deletion of ISCg2 family (ISCg1a, ISCg1b, ISCg1c, ISCg1e) This study
  WJ005 ATCC 13032 with in frame deletion of ISCg2 family (ISCg2b) This study
  WJ006 ATCC 13032 with in frame deletion of ISCg2 family (ISCg2b, ISCg2c) This study
  WJ007 ATCC 13032 with in frame deletion of ISCg2 family (ISCg2b, ISCg2c, ISCg2e) This study
  WJ008 ATCC 13032 with in frame deletion of ISCg2 family (ISCg2b, ISCg2c, ISCg2e, ISCg2f) This study
Plasmids   
 pCES208 E. coli—C. glutamicum shuttle vector, Kmr [45]
 pCES-NMCS pCES208 derivative; MCS and rrn terminator, Kmr [6]
 pH36M2 pCES208 derivative; PH36, PorB signal sequence, codon-optimized M18 scFv gene (opt) [5]
 pCES-H36-porBss pCES208 derivative; PH36, porinB signal sequence, Kmr This study
 pCES-H36-porBss-Amy pCES208 derivative; PH36, porinB signal sequence amylase, Kmr This study
 pCES-H36-porBss-IS-Amy pCES208 derivative; PH36, IS30 inserted porinB signal sequence, amylase, Kmr This study
 pCES-H36-GFP pCES208 derivative; PH36, eGFP, Kmr [46]
 pCnCAB phaCABC gene expression [41]
 pCES-H36-PhaC pCES208 derivative; PH36, phaC, Kmr This study
 pCES-H36-PhaCA pCES208 derivative; PH36, phaCA, Kmr This study
 pCES-H36-PhaCAB pCES208 derivative; PH36, phaCAB, Kmr This study
 pHGmut pCES208 derivative, Glu89Gln/Δ452-466 gene [26]
 pK19mobsacB Mobilizable vector, Kmr [42]
 pK19-ISCg1a pK19mobsacB derivative; flanking region of ISCg1a This study
 pK19-ISCg1b pK19mobsacB derivative; flanking region of ISCg1b This study
 pK19-ISCg1c pK19mobsacB derivative; flanking region of ISCg1c This study
 pK19-ISCg1e pK19mobsacB derivative; flanking region of ISCg1e This study
 pK19-ISCg2b pK19mobsacB derivative; flanking region of ISCg2b This study
 pK19-ISCg2c pK19mobsacB derivative; flanking region of ISCg2c This study
 pK19-ISCg2e pK19mobsacB derivative; flanking region of ISCg2e This study
 pK19-ISCg2f pK19mobsacB derivative; flanking region of ISCg2f This study
 pJET1.2/blunt Commercial cloning vector Thermob
  1. aNew England Biolabs, Beverly, MA, USA
  2. bThermo fisher scientific, Waltham, MA, USA