|
Strain
| | |
|
E. coli
| | |
|
XL1-blue
|
recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proABlacIq ZΔM15 Tn10 (Tet
r
)]
|
Stratagenea
|
|
C. glutamicum
| | |
|
ATCC 13032
|
Biotin-auxotrophic wild type
|
ATCC
|
|
WJ001
|
ATCC 13032 with in frame deletion of ISCg2 family (ISCg1a)
|
This study
|
|
WJ002
|
ATCC 13032 with in frame deletion of ISCg2 family (ISCg1a, ISCg1b)
|
This study
|
|
WJ003
|
ATCC 13032 with in frame deletion of ISCg2 family (ISCg1a, ISCg1b, ISCg1c)
|
This study
|
|
WJ004
|
ATCC 13032 with in frame deletion of ISCg2 family (ISCg1a, ISCg1b, ISCg1c, ISCg1e)
|
This study
|
|
WJ005
|
ATCC 13032 with in frame deletion of ISCg2 family (ISCg2b)
|
This study
|
|
WJ006
|
ATCC 13032 with in frame deletion of ISCg2 family (ISCg2b, ISCg2c)
|
This study
|
|
WJ007
|
ATCC 13032 with in frame deletion of ISCg2 family (ISCg2b, ISCg2c, ISCg2e)
|
This study
|
|
WJ008
|
ATCC 13032 with in frame deletion of ISCg2 family (ISCg2b, ISCg2c, ISCg2e, ISCg2f)
|
This study
|
|
Plasmids
| | |
|
pCES208
|
E. coli—C. glutamicum shuttle vector, Kmr
|
[45]
|
|
pCES-NMCS
|
pCES208 derivative; MCS and rrn terminator, Kmr
|
[6]
|
|
pH36M2
|
pCES208 derivative; PH36, PorB signal sequence, codon-optimized M18 scFv gene (opt)
|
[5]
|
|
pCES-H36-porBss
|
pCES208 derivative; PH36, porinB signal sequence, Kmr
|
This study
|
|
pCES-H36-porBss-Amy
|
pCES208 derivative; PH36, porinB signal sequence amylase, Kmr
|
This study
|
|
pCES-H36-porBss-IS-Amy
|
pCES208 derivative; PH36, IS30 inserted porinB signal sequence, amylase, Kmr
|
This study
|
|
pCES-H36-GFP
|
pCES208 derivative; PH36, eGFP, Kmr
|
[46]
|
|
pCnCAB
|
phaCABC gene expression
|
[41]
|
|
pCES-H36-PhaC
|
pCES208 derivative; PH36, phaC, Kmr
|
This study
|
|
pCES-H36-PhaCA
|
pCES208 derivative; PH36, phaCA, Kmr
|
This study
|
|
pCES-H36-PhaCAB
|
pCES208 derivative; PH36, phaCAB, Kmr
|
This study
|
|
pHGmut
|
pCES208 derivative, Glu89Gln/Δ452-466 gene
|
[26]
|
|
pK19mobsacB
|
Mobilizable vector, Kmr
|
[42]
|
|
pK19-∆ISCg1a
|
pK19mobsacB derivative; flanking region of ISCg1a
|
This study
|
|
pK19-∆ISCg1b
|
pK19mobsacB derivative; flanking region of ISCg1b
|
This study
|
|
pK19-∆ISCg1c
|
pK19mobsacB derivative; flanking region of ISCg1c
|
This study
|
|
pK19-∆ISCg1e
|
pK19mobsacB derivative; flanking region of ISCg1e
|
This study
|
|
pK19-∆ISCg2b
|
pK19mobsacB derivative; flanking region of ISCg2b
|
This study
|
|
pK19-∆ISCg2c
|
pK19mobsacB derivative; flanking region of ISCg2c
|
This study
|
|
pK19-∆ISCg2e
|
pK19mobsacB derivative; flanking region of ISCg2e
|
This study
|
|
pK19-∆ISCg2f
|
pK19mobsacB derivative; flanking region of ISCg2f
|
This study
|
|
pJET1.2/blunt
|
Commercial cloning vector
|
Thermob
|
